Uncovering general principles connecting neural circuits to behaviour requires comparative brain analyses across species. To advance these efforts, we developed a standardised workflow and a unified brain atlas platform for the quantitative comparison of neural structures between two teleost larvae, medaka (Oryzias latipes) and zebrafish (Danio rerio). Crucially, the platform seamlessly integrates user-generated image volumes, facilitating quantitative comparisons of anatomical, transcriptional, and functional data across species and laboratories.
Utilising these integrated tools, we identified regional anatomical differences and differentially expressed genes between the species. We further demonstrated functionally distinct telencephalic regions in both species that exhibit divergent responses to social stimuli, suggesting an evolutionary divergence in social processing mechanisms. This web-based, interactive atlas platform promotes data exchange and knowledge sharing within the teleost community.
Its scalable design permits effortless multi-group contributions, enabling expansion into a continuously growing, globally accessible database. We are grateful to the Engert lab and the Fishman lab at Harvard University for experimental and conceptual support. We appreciate the Bellono lab in Harvard University and Alexander Aulehla in EMBL for experimental support in making transgenic lines.
We acknowledge Epoch for experimental support in the generation of transgenic lines. We thank Armin Bahl in University of Konstanz for experimental support and kind suggestions in brain registration. We appreciate the Harvard Centre for Biological Imaging (HCBI) for microscopic imaging.
We acknowledge Iris Odstrcil for the help in backfill experiments. We appreciate Erin Yue Song for helping HCR experiments. We thank the Higashijima lab in the National Institute of Basic Biology for sharing the Tg (Glut:GFP) line.
Florian Engert received funding from the National Institutes of Health (U19NS104653 and 1R01NS124017-01), DoD (W911NF2420112) and the Simons Foundation (SCGB 542973 and NC-GB-CULM-00003241-02). Yasuko Isoe acknowledges the Uehara Memorial Foundation and Japan Society for Promoting Science for fellowship funding. Visual and Data-Centric Computing, Zuse Institute Berlin, Takustraße 7, Berlin, Germany Sumit Kumar Vohra, Hans-Christian Hege & Daniel Baum Research group behavioral neuroscience, Leibniz-Institut für Neurobiologie (LIN), Brenneckestraße 6, Magdeburg, Germany Centre for Organismal Studies (COS) Heidelberg, Heidelberg University, Im Neuenheimer Feld 230, Heidelberg, Germany Tinatini Tavhelidse-Suck & Joachim Wittbrodt Developmental Biology Unit, European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstraße 1, Heidelberg, Germany Department of Molecular and Cellular Biology, Faculty of Arts and Sciences, Harvard University, Cambridge, Massachusetts, USA Jonathan Boulanger-Weill, Jeff W.
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