Acute effects of blood flow restriction on phase-resolved electromyographic amplitude and whole-cycle agonist-antagonist co-contraction during incremental cycling

Blood flow restriction (BFR) can modify surface electromyographic (sEMG) amplitude during exercise, but it remains unclear whether these changes differ between the power and recovery phases of cycling and whether whole-cycle temporal and co-contraction descriptors also change. Because sEMG does not directly measure central drive or motor-unit behavior, the present study evaluated functional myoelectric outcomes rather than neural mechanisms. Forty recreationally active men completed incremental cycling sessions with BFR and under control conditions (CON) in a randomized crossover design.

Surface EMG was recorded from the rectus femoris (RF), vastus medialis (VM), vastus lateralis (VL), biceps femoris (BF), medial gastrocnemius (MG), and tibialis anterior (TA). Session-peak-normalized root mean square (RMS) amplitude was analyzed separately during the power (0°–180°) and recovery (180°–360°) phases. Median frequency (MF), threshold-defined activation duration, time-to-peak activation, and agonist-antagonist co-contraction indices were calculated over the complete pedal cycle.

Repeated-measures analyses examined condition, exercise stage, and phase where appropriate. BFR was associated with greater session-peak-normalized RMS amplitude in all muscles during both phases (all p